Journal: Scientific Reports

Article Title: Alpha-1 microglobulin as a potential therapeutic candidate for treatment of hypertension and oxidative stress in the STOX1 preeclampsia mouse model

doi: 10.1038/s41598-019-44639-9

Figure Lengend Snippet: Human rA1M protects kidney function but no effect on cardiac hypertrophy. ( a ) Albumin/Creatinine ratio (ACR) analysis of urine from pregnant females showed an increase of ACR at late-gestation in the preeclamptic females, which was absent in the rA1M-treated group. Shown is the mean ACR normalised to Ctrl-buff values for each gestation period, and n = number of females analysed. ( b ) Gene expression levels for HO-1 , CAT and SOD2 , normalised to the HPRT -gene levels in kidney from PE-buff and PE-A1M females, demonstrating significant reduction of HO-1 and CAT expression (*p = 0.04; **p = 0.04) after rA1M-treatment. The line represents the median and n = number of females analysed. ( c ) Preeclamptic females showed increased heart weight compared to Ctrl-buff at 17.5 dpc (*p = 0.002), which could not be alleviated by rA1M treatment (PE-A1M vs Ctrl-buff; **p = 0.008). Control groups showed similar heart weight as non-pregnant females. The line represents the median and n = number of females analysed.

Article Snippet: Real-time PCR was performed for HO-1 , CAT , SOD2 , with hypoxanthine phosphoribosyltransferase ( HPRT) as endogenous control, using TaqMan Gene Expression Assays specific for mouse ( HO-1 – Mm00516005_m1, CAT – Mm00437992_m1, SOD2 – Mm01313000_m1, HPRT – Mm01545399_m1, Life Technologies).

Techniques: Gene Expression, Expressing, Control

Journal: eLife

Article Title: Plural molecular and cellular mechanisms of pore domain KCNQ2 encephalopathy

doi: 10.7554/eLife.91204

Figure Lengend Snippet:

Article Snippet: Antibody , Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific , Jackson ImmunoResearch , Code: 211-032-171 Lot #: 97224 RRID: AB_2339149 , WB (1:5000).

Techniques: Transfection, Construct, Mutagenesis, Recombinant, Expressing, Plasmid Preparation, Gene Expression, Real-time Polymerase Chain Reaction, Sequencing, Western Blot, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Incubation, Software

Journal: EMBO Reports

Article Title: Promyelocytic leukemia protein targets MK2 to promote cytotoxicity

doi: 10.15252/embr.202052254

Figure Lengend Snippet:

Article Snippet: Peroxidase IgG Fraction Monoclonal Mouse Anti‐Goat IgG, light chain specific , Jackson ImmunoResearch , 205‐032‐176, RRID:AB_2339056 , WB 1:20,000.

Techniques: Generated, Transgenic Assay, Recombinant, Plasmid Preparation, Blocking Assay, Transfection, Antibody Labeling, Software, Cell Viability Assay